SKUMPIYA (Cotinus coggygria Scop.) O‘SIMLIGINI IN VITRO SHAROITIDA MIKROKLONAL KO‘PAYTIRISH TEXNOLOGIYASINI TAKOMILLASHTIRISH
Keywords:
Cotinus coggygria, in vitro, mikroklonal ko‘paytirish, sterilizatsiya, NaOClAbstract
Respublikamizda “Yashil makon” umummilliy loyihasi doirasida manzarali daraxt va buta o‘simliklarini qisqa muddatda ko‘paytirish dolzarb vazifalardan biri hisoblanadi. Mazkur tadqiqotda Cotinus coggygria Scop. turini in vitro sharoitida mikroklonal ko‘paytirishning samarali texnologiyasini ishlab chiqish maqsadida sterilizatsiya rejimlari hamda oziqa muhitlari o‘rganildi. Tadqiqot davomida NaOCl ning 0,3; 0,5 va 0,7 % konsentratsiyalari 10, 15 va 20 daqiqalik ekspozitsiya bilan sinovdan o‘tkazildi. Eksplantlarning rivojlanishi Murashige and Skoog (MS), Driver va Kuniyuki (DKW) hamda Woody Plant Medium (WPM) oziqa muhitlarida baholandi.
Natijalarga ko‘ra, 0,5 % NaOCl eritmasida 10 daqiqa sterilizatsiya qilish 88 % steril va yashovchan eksplant olish imkonini berdi. Oziqa muhitlari orasida MS + 1,0 mg/l BAP varianti novda hosil bo‘lishi, poya uzunligi hamda mikroko‘paytirish koeffitsienti bo‘yicha eng yuqori natijalarni qayd etdi. Tadqiqot natijalari C. coggygria ni ommaviy mikroklonal ko‘paytirish texnologiyasini ishlab chiqish hamda manzarali ko‘chatlar ishlab chiqarishga joriy etish imkonini beradi
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References
1. Begon M., Townsend C.R., Harper J.L. Ecology: From Individuals to Ecosystems. – 4th ed. – Oxford: Blackwell Publishing, 2006. – 738 p.
2. Benson E.E. In Vitro Plant Recalcitrance // In Vitro Cellular & Developmental Biology – Plant. – 2000. – Vol. 36. – P. 141–148.
3. Berdiyev E.T., Saloxiddinov G‘.M., Turdiyev S.A. O‘rmon tuzish: o‘quv qo‘llanma. – Toshkent: ToshDAU tahririyat-nashriyot bo‘limi, 2016. – 155 b.
4. Beydeman I.N. Uchebno-metodicheskiy kompleks distsipliny «Metody fenomonitoringa»: kurs lektsiy. – Yekaterinburg, 2008.
5. Bonga J.M., Aderkas P.V. In Vitro Culture of Trees. – Dordrecht: Kluwer Academic Publishers, 1992. – 236 p.
6. Debnath S.C., da Silva J.A.T. Micropropagation of berry crops // Plant Cell, Tissue and Organ Culture. – 2007. – Vol. 91. – P. 1–17.
7. Dospexov B.A. Metodika polevogo opyta. – 5-e izd., pererab. i dop. – Moskva: Agropromizdat, 2011. – 351 s.
8. Driver J.A., Kuniyuki A.H. In vitro propagation of Paradox walnut rootstock // HortScience. – 1984. – Vol. 19. – P. 507–509.
9. George E.F., Hall M.A., De Klerk G.J. Plant Propagation by Tissue Culture. – 3rd ed. – Dordrecht: Springer, 2008. – 501 p.
10. Lloyd G., McCown B. Commercially feasible micropropagation of mountain laurel by use of shoot-tip culture // Proceedings of the International Plant Propagators' Society. – 1980. – Vol. 30. – P. 421–427.
11. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiologia Plantarum. – 1962. – Vol. 15. – P. 473–497.
12. Rout G.R., Mohapatra A., Jain S.M. Tissue culture of ornamental pot plant // Biotechnology Advances. – 2006. – Vol. 24. – P. 531–560.
13. Thorpe T.A. History of plant tissue culture // Molecular Biotechnology. – 2007. – Vol. 37. – P. 169–180.
14. Trigiano R.N., Gray D.J. Plant Tissue Culture, Development and Biotechnology. – Boca Raton: CRC Press, 2016. – 608 p.
15. Xonazarov A.A. O‘zbekistonda o‘rmonzorlar barpo qilish asoslari. – Toshkent, 2002. – 48 b.
